THE UNIVERSITY OF CALGARY

FACULTY OF SCIENCE

MAKE-UP EXAMINATION

BIOLOGY 403

March 22, 1992 TIME: 1 HOUR

TOTAL POINTS: 100

(VALUE) QUESTION

(20) 1. Discuss the regulation of the chicken ovalbumin gene. Include in your answer an analysis of experiments designed to clarify the molecular mechanisms involved in regulation.

(60) 2. Reconstruct the series of events beginning with oogenesis that leads to muscle cell determination and differentiation in mesoderm of Xenopus. Be complete. If in doubt, include all regulatory events that might bear upon development of muscle in this organism.

(20) 3. Interpret the following experiment conducted with Drosophila. Double mutants of oskar with each of the following mutant genes were constructed: hunchback, Krüppel, and knirps. As a result, hunchback is normal, Krüppel expression is extended posteriorly, and knirps is not expressed. What do these results tell you about the relationships between oskar and these three genes?