THE UNIVERSITY OF CALGARY
FACULTY OF SCIENCE
April, 1993 TIME: 2 hours
TOTAL POINTS: 100
(20) 1. When Drosophila females are homozygous for the mutant
gene eggnog (egn), cellularization of the blastoderm does
not occur in their progeny. However, all progeny of a mating between egn+/egn- males and egn+/egn- females cleave normally.
A transgenic Drosophila strain was produced in which the 100 nucleotides
upstream of egn+ were fused to the luciferase
reporter gene. What experiments would you perform to identify the trans-acting
factors that regulate transcription of egn+?
(20 ) 2. Discuss the roles of the pair-rule genes in patterning of the
Drosophila embryo. Identify the genes involved and discuss their
individual roles in as much detail as you can. Cite experimental evidence
(60) 3. Reconstruct the series of events beginning with oogenesis that
leads to muscle cell determination and differentiation in mesoderm of Xenopus.
Be complete. If in doubt, include all regulatory events that might bear
upon development of muscle in this organism.