University of Calgary

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Gou J, Hao F, Huang C, Kwon M, Li H, Chen F, Li C, Liu C, Zhang W, Ro DK, Tang H, Zhang Y (2017) Discovery of non-stereoselective cytochrome P450 catalyzing either 8alpha- or 8beta-hydroxylation of germacrene A acid from the Chinese medicinal plant, Inula hupehensis. Plant J., In Press

Hurd MC, Kwon M, *Ro DK (2017) Functional identification of a Lippia dulcis bornyl diphosphate synthase that contains a duplicated, inhibitory arginine-rich motif. Biochem. Biophys. Res. Commun. 490:963-968

Liu X, Liu Y, Huang P, Ma Y, Qing Z, Tang Q, Cao H, Cheng P, Zheng Y, Yuan Z, Zhou Y, Liu J, Tang Z, Zhuo Y, Zhang Y, Yu L, Huang J, Yang P, Peng Q, Zhang J, Jiang W, Zhang Z, Lin K, Ro DK, Chen X, Xiong X, Shang Y, Huang S, Zeng J (2017) The genome of the medicinal plant Macleaya cordata provides new insights into benzylisoquinoline alkaloids metabolism. Molecular Plant, 10:975-989

Zhong Y, Xue X, Liu Z, Ma Y, Zeng K, Han L, Qi J, Ro DK, Bak S, Huang S, Zhou Y (2017) Regulated Glucosylation of Bitter Triterpenoid In Cucumber by the Udp-Glucosyltransferase UGT73AM3. Molecular Plant, 10:1000-1003

Pelot KA, Mitchell R, Kwon M, Hagelthorn DM, Wardman JF, Chiang A, Bohlmann J, Ro DK, Zerbe P (2017) Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase. Plant J., 89: 885-897


Zhou Y, Ma Y, Zeng J, Duan L,, Xue X, Wang H, Lin T, Liu Z, Zeng K, Zhong Y, Zhang S, Hu Q, Liu M, Zhang H, Reed J, Moses T, Liu X, Huang P, Qing Z, Liu X, Tu P, Kuang H, Zhang Z, Osbourn A, Ro DK, *Shang Y, *Huang S (2016) Convergence and divergence of bitterness biosynthesis and regulation in Cucurbitaceae. Nature Plants, 2:16183

Nguyen TD, Faraldos JA, Vardakou M, Salmon M, O'Maille PE, Ro DK (2016) Discovery of germacrene A synthases in Barnadesia spinosa: The first committed step in sesquiterpene lactone biosynthesis in the basal member of the Asteraceae. Biochem. Biophys. Res. Commun., 479:622-627

Ro DK, Qu Y, Kwon M (2016) Synthetic Biology of Plant Natural Products, in Plant Specialized Metabolism: Genomics, Biochemistry, and Biological Functions (edited by M. E. Maffei and G.I. Arimura), Chapter 13, p315-360, CRC Press

Kwon M, Kwon EJG, Ro DK (2016) cis-Prenyltransferase and polymer analysis from a natural rubber perspective. Mehtods Enzymol., 576:121-145

Aschenbrenner AK, Kwon M, Conrad J, Ro DK, Spring, O. (2016) Identification and characterization of bisabolene synthases from linear glandular trichomes of sunflower (Helianthus annuus L., Asteraceae). Phytochemistry, 124:29-37


Chakrabarty R, Qu Y, *Ro DK (2015) Silencing the lettuce homologs of small rubber particle protein does not influence natural rubber biosynthesis in lettuce (Lactuca sativa). Phytochemistry, 113:121-129

Qu Y, Chakrabarty R, Tran HT, Kwon EJG, Kwon M, Nguyen TD, *Ro DK (2015) A lettuce (Lactuca sativa) homolog of human Nogo-B receptor interacts with cis-prenyltransferase and is necessary for natural rubber biosynthesis. J. Biol. Chem. 290:1898-1914


Kwon M, Cochrane SA, Vederas JC, *Ro DK (2014) Molecular cloning and characterization of drimenol synthase from valerian plant (Valeriana officinalis) FEBS Letters 588:4597-4603

Ferraro K,, Jin AL, Nguyen TD, Reinecke DM, Ozga JA, *Ro DK (2014) Characterization of proanthocyanidin metabolism in pea (Pisum sativum) seeds. BMC Plant Biol. 14:238

Giannakopoulou A, Schornack S, Bozkurt TO, Haart D, Ro DK, Faraldos JA, Kamoun S, *O'Maille PE (2014) Variation in capsidiol sensitivity between Phytophthora infestans and Phytophthora capsici is consistent with their pathogen host range. PLoS One 9:e107462

Son YJ, Kwon M, *Ro DK, *Kim SU (2014) Enantioselective microbial synthesis of the indigenous natural product, (-)-a-bisabolol, by a sesquiterpene synthase from chamomile (Matricaria recutita). Biochem. J. 463:239-248


Xiao M, Zhang Y, Chen X, Lee EJ, Barber CJ, Chakrabarty R, Desgagne-Penix I, Haslam TM, Kim YB, Liu E, MacNevin G, Masada-Atsumi S, Reed DW, Stout JM, Zerbe P, Zhang Y, Bohlmann J, Covello PS, De Luca V, Page JE, Ro DK, Martin VJ, Facchini PJ, *Sensen CW. (2013) Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest. J. Biotechnology. 166:122-134


Pickel, B, Drew DP, Manzcak T, Weitzel C, *Simonsen HT, *Ro DK(2012) Identification and characterization of a kunzeaol synthase from Thapsia garganica: Implications for the biosynthesis of the pharmaceutical thapsigargin. Biochem. J. 448:261-271

Nguyen DT, MacNevin G, *Ro DK (2012) De novo synthesis of high-value sesquiterpenoids in yeast. Methods Enzymol. 517:261-278

Attia M, Kim SU, *Ro DK(2012) Molecular characterization of (+)-epi-alpha-bisabolol synthase from Lippia dulcis - the entry point enzyme for the natural sweetener, hernandulcin, biosynthesis. Arch. Biochem. Biophys. 527:37-44

Pyle BW, Tran HT, Pickel B, Haslam TM, Gao Z, MacNevin G, Vederas JC, Kim SU, *Ro, D.K. (2012) Enzymatic synthesis of valerena-4,7(11)-diene by a unique sesquiterpene synthase from valerian plant (Valeriana officinalis). FEBS J. 279:3136-3146

Rowe HC, Ro DK, *Rieseberg LH (2012) Response of sunflower (Helianthus annuus L.) leaf surface defenses to exogenous methyl jasmonate. PLoS One. 7:e37191

Facchini PJ, Bohlmann J, Covello PS, De Luca V, Mahadevan R, Page JE, Ro DK, Sensen CW, Storms R, *Martin VJJ (2012) Synthetic biosystems for the production of high-value plant metabolites. Trends Biotechnol. 30:127-131


Ikezawa N, Gopfert JC, Nguyen DT, Kim SU, O'Maille PE, Spring O, *Ro DK (2011) Lettuce costunolide synthase (CYP71BL2) and its homolog (CYP71BL1) from sunflower catalyze distinct regio- and stereoselective hydroxylations in sesquiterpene lactone metabolism. J. Biol. Chem. 286:21601-21611

Barriuso J, Nguyen DT, Li J, Roberts J, MacNevin G, Chaytor JL, Marcus S, *Vederas JC, *Ro DK (2011) Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a single cytochrome P450 monooxygenase, LovA. J. Am. Chem. Soc. 133: 8078-8081

Ro DK Terpenoid Biosynthesis (Chapter 8, 217-240), Plant Metabolism and Biotechnology, 2011, Edited by Hiroshi Ashihara, Alan Crozier and Atsushi Komamine, John Wiley & Sons


Nguyen DT, Gopfert JC, Ikezawa N, MacNevin G, Kathiresan M, Conrad J, Spring O, *Ro DK (2010) Biochemical Conservation and Evolution of Germacrene A Oxidase in Asteraceae J. Biol. Chem. 285:16588-16598.


*Gopfert JC, MacNevin G, Ro DK, Spring O (2009) Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes BMC Plant Biology 9:86

Prior to University of Calgary

Ro, D.K., Ouellet, M., Paradise, E. M., Burd, H., Eng, D., Paddon, C., Newman, J. D. and *Keasling, J.D. (2008) Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce the anti-malarial drug precursor, artemisinic acid. BMC Biotechnol. 8:83.

Chang, M.C.Y., Eachus, R.A., Trieu, W., Ro, D.K. and *Keasling J.D. (2007) Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nature Chem. Biol. 3: 274-277.

Shiba, Y. Paradiase, E.M., Kirby, J., Ro, D.K., *Keasling, J.D. (2007) Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids. Metabolic Engineering 9: 160-168

Ro, D.K., Paradise, E.M., Ouellet, M., Fisher, K.J., Newman, K.L., Ndungu, J.M., Chang, M.C.Y., Ham, T.S., Eachus, R.A., Ho, K.A., Shiba, Y., Sarpong, R., *Keasling, J.D. (2006) Production of the anti-malarial drug precursor artemisinic acid in engineered yeast. Nature 440: 940-943

Ro, D.K. and *Bohlmann, J. (2006) Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): Functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1). Phytochemistry 67: 1572-1578

Ro, D.K., Ehlting, J., Keeling, C.I., Lin, R., Mattheus, N, and *Bohlmann, J. (2006) Microarray expression profiling and functional characterization of AtTPS genes: Duplicated Arabidopsis thaliana sesquiterpene synthase genes At4g13280 and At4g13300 encode root-specific and wound-inducible (Z)-gamma-bisabolene synthases. Arch. Biochem. Biophysics. 448: 104-116

Ro, D.K., Arimura, G.I., Lau, S, Piers, E., and *Bohlmann, J. (2005) Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multi-functional, multi-substrate cytochrome P450 monooxygenase. Proc. Nat. Acad. Sci. USA. 102: 8060-8065

Chen, F., Ro, D.K., Petri, J., Gershenzon, J., Bohlmann, J., Pichersky, E., and *Tholl, D. (2004) Characterization of a root-specific arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole. Plant Physiol. 135: 1956-1966

Ro, D.K., and *Douglas, C.J. (2004) Reconstitution of the entry point of plant phenylpropanoid metabolism in yeast (Saccharomyces cerevisiae): Implications for control of metabolic flux into the phenylpropanoid pathway. J. Biol. Chem. 279: 2600-2607

Ro, D.K., Ehlting, J., and *Douglas, C.J. (2002) Cloning, functional expression, and subcellular localization of multiple NADPH-cytochrome P450 reductases from hybrid poplar. Plant Physiol. 130: 1837-1851

Ro, D.K., Mah, N., Ellis, B.E., and *Douglas, C.J. (2001) Functional characterization and subcellular localization of poplar (Populus trichocarpa x Populus deltoides) cinnamate 4-hydroxylase. Plant Physiol. 126: 317-329