Permeation Kinetics of Dimethyl Sulfoxide in Articular
Cartilage
K Muldrew, B Sykes, NS Schachar and LE McGann
Key Words: cryoprotectant diffusion, tissue cryopreservation, proton
NMR, articular cartilage
1H-NMR was used to monitor the concentration of dimethyl sulfoxide in
articular cartilage on patellae from pigs as a function of time and
temperature. The resulting diffusion coefficient was
1.12x10-5 cm2/s
at 23°C with an Arrhenius activation energy of 0.95 kcal/mol.
Concentration profiles in cartilage of various thicknesses were
calculated as a practical aid for the design of protocols to
cryopreserve articular cartilage.
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This document last updated on 11/17/95.