Kit 1A

Transposons in the Genome of S. Typhimurium LT2

Insertions of transposons into and between known genes of S. typhimurium. This includes over 1,000 strains, including insertions of Tn10, MudA, MudJ, MudK, MudP22, and others. Strains were provided by many investigators. (Ref: Altman, E. et. al. 1996. Transposons currently in use in genetic analysis of Salmonella species. In E.coli and Salmonella, 2nd Edn., Ed. Neidhardt, F.C. ASM Press.1996., Volume 2 Chapter 141, Table 2 and Sanderson, K.E. and Roth, J. R. 1983. Microbiol. Rev. 47:410-453)

Link to table of strains of Salmonella Typhimurium LT2 with transposons inserted into the genome (a):

Kit 1A Strain Table

Kit 1A Table Footnotes:

(a) These mutant strains of S. typhimurium LT2 have been isolated in many different laboratories; the largest number come from the laboratory of J.R. Roth. The strains have been provided to the SGSC, where they are maintained and are available for circulation to any investigator.

(b) The centisome (CS) indicates the location on the map at which the transposon listed for the strain is inserted. This location is based on the genetic map of S. typhimurium (see E.coli and Salmonella, 2nd Edn., Ed. Neidhardt, F.C. ASM Press. 1996., Volume 2 Chapter 110). Since this map is based on physical distances, the locations of some genes are different from those in earlier versions of the linkage map, which were based on genetic distances indicated in minutes (of transfer time in conjugation) (Ref: Sanderson, K.E. and Roth, J.R. 1988. Microbiol. Rev. 52:485-532). The locations of transposon insertions with the same CS number are frequently but not always known with respect to each other. For example, at CS2, the insertion in araB is counterclockwise from the insertions in leu according to the genetic map (displayed as a circle in E.coli and Salmonella, 2nd Edn., Ed. Neidhardt, F.C. ASM Press. 1996., Volume 2 Chapter 128), but the exact locations of the insertions designated zac are not known with respect to each other.

(c) The gene into which the transposon is inserted and its allele number are at the left. Insertions into a gene cause loss of gene function; e.g. insertions in thr result in a Thr(-) (threonine requiring) phenotype. Transposon insertions which cause no detectable change in phenotype are designated zxx followed by an allele number. If these insertions have been located on the genetic map (e.g., through linkage to known genes), this designation is changed according to the system of Hong and Ames (1971. Proc. Natl. Acad. Sci. 68:3158-3162); thus insertions at 0 CS are zaa, insertions as 1 CS are zab etc. The allele number of the mutation produced by the insertion is never changed even if the map position must be changed as data are refined; the second two letters of the designation may be changed to indicate the new map location, but they are frequently not changed, and thus, though these letters are helpful in locating an insertion on the genetic map, they do not always indicate the exact position.

The transposon inserted in each strain is indicated after the double colon; each transposon type is described in more detail in the text. Strains with insertions at the same CS are listed in alphabetical order of the transposon type. The antibiotic resistance associated with each transposon is indicated in parentheses after the transposon. Some of the data used to locate the site of the transposon insertion are given after the insertion. The percentage is the joint transduction by phage P22 between the transposon and the listed gene (%ND, percentage not determined).

(d) The laboratory of origin of the strain is usually indicated by the strain designation, as follows: AK, A. Kukral, c/o R. Maurer, Case Western Reserve University; CBS, C. Berg, University of Conneticut; CH, C. Higgins, Oxford University; CJD, C. Dorman, University of Dundee; DA, D. Anton, Argentina (or D. Andersson, Upsala); DB, D. Berkowitz, Massachussetts Institue of Technology (MIT); DU, R. Burns, Duke University; EB, E. Barrett, University of California at Davis (UC Davis); GW, G.Walker, MIT; HSK, H.S. Kwan, Chinese University of Hong Kong; HU, P. Ayling, University of Hull; JF, J.W. Foster, University of South Alabama; JL, J. Ingraham, UC Davis; KK, K. Kutsukake, Hiroshima University; KP, J. Neuhard, Copenhagen; KR, R. Kelln, University of Regina; KS, W.W. Kay, University of Victoria, MM, M. Maguire, Case Western Reserve University; MS, S. Maloy, University of Illinois; NK, N. Kleckner, Harvard University; NR, D. Podger, Australia; PH, P Hallenbech, University of Montreal; PM, P. Margolin, c/o SGSC; PP, P.W. Postma, Netherlands; RL, T. VanDyk, DuPont Co.; RT, R. Jeter, Texas Tech University; SA, K.Sanderson, University of Calgary; SH, P.H. Makela, Finland; SK, S. Kustu, UC Berkeley; SL, B.A.D. Stocker, Stanford University; SMS, T. VanDyk, DuPont Co; ST, D Koshland, UC Berkeley; SV, J. Casadesus, University of Seville; TA, B.N. Ames, UC Berkeley; TE, T. Elliot, University of Alabama; TL, L. Csonka, Purdue University; TN, C. Miller, University of Illinois; TS, T. Palva, Swedish University of Agriculrural Science; TT, J. Roth, University of Utah.

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