KIT 2A

Lipopolysaccaride Mutant Kit

Set of mutant strains of S. typhimurium LT2, representing defects in the lipopolysaccharide. The strains were constructed as an isogenic set of mutants with wild type LT2 by Roantree et al. 1977. J. Gen. Microbiol. 103:223-234, and Sanderson et al. 1974. Can. J. Microbiol. 20: 1135-1145.

Strain

Number

SGSC

Number

Chemotype

of LPS

Partial

Genotype(c)

SL3770 (a)

225

smooth

rfa (+)

SA1627(his-642)

-

Ra

rfb-his deletion

SL3749 (a)

228

Ra

rfaL446

SL733

389

Rb1

rfaK953

SL3750 (a)

229

Rb2

rfaJ417

SL3748 (a)

227

Rb3

rfaI432

SL3769 (a)

231

Rd1

rfaG471

SL3789 (a)

230

Rd2

rfaF511

SL1306

163

Rc

galE503

SA1377 (b)

-

Re

rfaC630

SL1102

258

Re

rfaE543

SL3600

358

Re

rfaD657

SL428

68

semi-rough (Ra with 1 side chain unit)

rfc-458

SL4807

356

mixture of Rc + smooth

rfaB707

 

(a) These strains are part of an isogenic set of mutants constructed by Roantree et al. 1977. J. Gen. Microbiol. 103:223-234.

(b) These strains are part of an isogenic set of mutants constructed by Sanderson et al. 1974. Can. J. Microbiol. 20: 1135-1145.

(c) A complete genotype is included when strains are shipped.

 

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