KIT 2B

Kits of Histidine Mutants

 

Kit 2B.1. Point Mutants in Genes of the Histidine Operon

These mutants were obtained from the laboratory of Philip E. Hartman, Dept. of Biology, The John Hopkins University, Baltimore, MD 21218. The mutant strains are described in Hartman, P.E., Z. Hartman, R.C. Stahl, and B.N. Ames. 1971. Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella. Advances in Genetics 16: 1-34. More recent references are given in K.E. Sanderson and J.R. Roth. 1983. Linkage map of Salmonella typhimurium, edition VI, Microbiological Revs. 47: 410-453, and in earlier editions of the linkage map. The strains should be referred to and published under the strain number and the genotype; the SGSC Number is only a filing number used by the Stock Centre.

 

Genotype
 

Strain

Number

SGSC

Number

Mutations

in his gene

Other

Mutations

Histidine

Enzyme Defect

SB4286

838

hisA478
 

Isomerase

SB8000

839

hisA3000

ara-9
 

SB4287

840

hisA329
   

SB4288

843

hisB482
 

Dehydrase-phosphatase

SB4289

844

hisB624
   

SB4290

845

hisB2778
   

TA939

846

hisC2499

hisO1242
 

Transaminase

SB5802

833

his802

ara-9

Dehydrogenase

TA376

847

hisD2121

hisO1242
   

SB4282

834

hisF41
 

PR-ATP-pyrophosphohydrolase

SB4283

835

hisF109
   

SB4284

836

hisF197
   

SB4285

837

hisF486
   

TR421

841

hisH2167
 

Amino-transferase

TR720

842

hisH2449
   

SB4281

832

hisI99
 

PR-AMP-hydrolase

 

Return to List of SGSC Kits


KIT 2B

Kits of Histidine Mutants

 

Kit 2B.2. Deletion Mutants in the Histidine Operon

These mutants were obtained from the laboratory of Philip E. Hartman, Dept. of Biology, The John Hopkins University, Baltimore, MD 21218. The mutant strains are described in Hartman, P.E., Z. Hartman, R.C. Stahl, and B.N. Ames. 1971. Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella. Advances in Genetics 16: 1-34. More recent references are given in K.E. Sanderson and J.R. Roth. 1983. Linkage map of Salmonella typhimurium, edition VI, Microbiological Revs. 47: 410-453, and in earlier editions of the linkage map. The strains should be referred to and published under the strain number and the genotype; the SGSC Number is only a filing number used by the Stock Centre.

   

Genotype
 

Strain

Number

SGSC

Number

Mutations

in his gene

Other

Mutations

SB5712

848

delhis(hisEIFAHBCD)712
 

SB4291

849

delhis(hisEI)640
 

SB4292

850

delhis(hisEI)659
 

SB4293

851

delhis(hisEIFAHB)612
 

SB8050

852

delhis(hisEIFAHB)3050
 

TA967

853

delhis(hisEIFA)2627
 

TA514

854

delhis(hisEIF)2315
 

TA947

855

delhis(hisEIFAHBC)2607
 

TA522

856

delhis(hisEIFAH)2323
 

SB4294

857

delhis(hisIF)135
 

SB5703

863

delhis(hisIF)703
 

SB4295

858

delhis(hisFA)645
 

SB4296

859

delhis(hisAH)134
 

TA973

860

delhis(hisAHB)2633
 

SL4024

861

delhis(hisFAHBCD)152
 

SB5644

862

delhis(hisFAHBCDG)644

gal-107

SB4297

864

delhis(hisHB)22
 

SB4298

865

delhis(hisCD)129
 

TA462

866

delhis(hisCD)2236
 

TA452

867

delhis(hisBCDG)2226
 

SB4299

868

delhis(hisCDG)63
 

SB4300

869

delhis(hisCDG)538
 

TA454

870

delhis(hisDG)2228
 

TR3066

871

delhis(hisG)8442
 

 

Return to List of SGSC Kits


KIT 2B

Kits of Histidine Mutants

 

Kit 2B.3. Set of F-Prime Strains with Mutations in the his Genes of E. coli K-12

These mutants were obtained from the laboratory of Philip E. Hartman, Dept. of Biology, The John Hopkins University, Baltimore, MD 21218. The mutant strains are described in Hartman, P.E., Z. Hartman, R.C. Stahl, and B.N. Ames. 1971. Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella. Advances in Genetics 16: 1-34. More recent references are given in K.E. Sanderson and J.R. Roth. 1983. Linkage map of Salmonella typhimurium, edition VI, Microbiological Revs. 47: 410-453, and in earlier editions of the linkage map. The strains should be referred to and published under the strain number and the genotype; the SGSC Number is only a filing number used by the Stock Centre.

This set of F-prime strains was constructed by the laboratories of P.E. Hartman and J.R. Roth from an F-prime carrying the his+ gene of E. coli, obtained from the Coli Genetic Stock Centre. Point mutants were isolated in the his genes of E. coli. The F-prime factors are maintained in a strain of S. typhimurium LT2. They can be used to do complementation tests with mutants of S. typhimurium to determine unknown alleles. They can be used in crosses into E.coli K-12 but the data are not as clear because of recombination between the his genes of the chromosome and of the F-prime. To determine the mutated gene of a His- mutant, grow that His- mutant overnight in broth with each of the F-prime strains shown below, then plate out on minimal medium to detect His+ recombinants.

 

Genotype

Strain

Number

SGSC

Number

Chromosome

(Salmonella genes)

F-Prime

(E.coli genes)

TA35

874

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' his+

TR84

875

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisE2414

TR79

876

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisIa2409

TR82

882

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisIab2412

TR78

877

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisF2408

TR76

878

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisA2406

TR75

879

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' Æhis (hisBH)2405

TR53

880

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisC2383

TA46

873

delhis (hisEIFAHBCD) 712 ser-821

/ F' hisDab2482

TG5701

881

delhis (hisEIFAHBCD) 712 ser-821 arg-501

/ F' hisG2416

TA34

872

delhis (hisEIFAHBCD) 712 ser-821

/ F' Æhis (hisHAFIE)2905

 

Return to List of SGSC Kits


SGSC Homepage - About the SGSC - On-Line Search - On-Line Order Form - Salmonella Sequences - Related Websites - Publications