KIT 3

Hfr Strains of S. typhimurium and S. abony

The F-factor of E. coli K-12 was transferred into S. typhimurium and S. abony, and a set of Hfr strains was isolated by Sanderson et al., 1972. Bacteriol. Rev. 36:603-637. These strains have been further described in Sanderson, 1996. E. coli and Salmonella, Cellular and Molecular Biology, edition 2, F. Neidhardt, editor, pps. 2406-2412, ASM Press.

These strains are described in the following Table and Figure:

Strain No.

SGSC No.

Species

Sex type

Source

Genotype(a)

Fin phenotype(b)

Phage sensitivity phenotype(c)

Point of Origin of transfer(d)

Genes transferred

Min

Direction

SA27

-

S. typhimurium

F(+)

SA1383

purE8 rfx-3054

-

rough

-

-

-

SA28

-

S. typhimurium

F(+)

SA1384

serA13 rfa-3058

-

rough

-

-

-

SA607

-

S. typhimurium

F(+)

-

serA15

-

smooth

-

-

-

SA627

-

S. typhimurium

F(+)

-

thrA9

-

smooth

-

-

-

SR305

-

S. typhimurium

HfrA

N. Zinder

hisD23 gal-50

+

smooth

82

CW

O-ilv-metA

SR315

-

S. typhimurium

FS21

N. Zinder

leu-256

+

smooth

30

CW

O-pyrF-trp

SU354

-

S. typhimurium

HfrB2

-

metA22 P22(+)

-

smooth

35

CW

O-tre-his

SU418

-

S. typhimurium

HfrB2

-

proA26 P22(+)

-

smooth

35

CW

O-tre-his

SU436

SA3307

S. typhimurium

HfrB3

SR297

hisD23 gal-50

+

smooth

72

CCW

O-argE-serA

SA458

-

S. typhimurium

HfrK1-1

SA28

serA13 rfa-3058

-

rough

79

CW

O-pyrE-ilv

SA464

-

S. typhimurium

HfrK1-2

SA28

serA13 rfa-3058

-

rough

79

CW

O-pyrE-ilv

SA486

-

S. typhimurium

HfrK3

SA28

serA13 rfa-3058

-

rough

61

CW

O-argE-ilv

SA534

-

S. typhimurium

HfrK4

SA28

serA13 rfa-3058

-

rough

-

CW

O-pyrB-thr

SA535

-

S. typhimurium

HfrK5

SA28

serA13 rfa-3058

-

rough

-

CW

O-metG-aroD

SA536

-

S. typhimurium

HfrK6

SA28

serA13 rfa-3058

-

rough

76

CW

O-xyl-cysE

SA540

-

S. typhimurium

HfrK2-1

SA27

purE8 rfx-3057

-

rough

79

CCW

O-cysE-xyl

SA639

-

S. typhimurium

HfrK1-6

SA622

serA150

-

rough

79

CW

O-pyrE-ilv

SA640

-

S. typhimurium

HfrK2-2

SA622

serA150

-

rough

79

CCW

O-cysE-xyl

SA642

-

S. typhimurium

HfrK1-7

SA622

serA150

-

rough

79

CW

O-pyrE-ilv

SA653

-

S. typhimurium

HfrK2-3

SA627

thrA9

-

rough

79

CCW

O-cysE-xyl

SA654

-

S. typhimurium

HfrK9

SA627

thrA9

-

smooth

40

CW

O-his-metG

SA722

-

S. typhimurium

HfrK10

SA607

serA15 pur-268

-

smooth

84

CCW

O-ilv-pyrE

SA828

-

S. typhimurium

HfrK25

SA804

serA15 rfx

-

rough

30

CW

O-trp-his

SA949

-

S. typhimurium

HfrK7

SA991

leuBCD39 ara-7

-

smooth

76

CW

O-xyl-cysE

SA952

-

S. typhimurium

HfrK14

SA991

leuBCD39 ara-7

-

smooth

80

CW

O-pyrE-ilv

SA955

-

S. typhimurium

HfrK20

SA28

serA13 rfa-3058

-

rough

15

CCW

O-purE-pro

SA962

-

S. typhimurium

HfrK16

SA991

leuBCD39 ara-7

-

smooth

41

CW

O-his-metG

SA965

-

S. typhimurium

HfrK17

SA991

leuBCD39 ara-7

-

smooth

10

CCW

O-pro-metA

SA966

-

S. typhimurium

HfrK19

SA991

leuBCD39 ara-7

-

smooth

98

CCW

O-purA-metA

SA967

-

S. typhimurium

HfrK1-8

SA991

leuBCD39 ara-7

-

rough

79

CW

O-pyrE-ilv

SA969

-

S. typhimurium

HfrK15

SA991

leuBCD39 ara-7

-

-

90

CCW

O-thi-argF

SA970

-

S. typhimurium

HfrK11

SA28

serA13 rfa-3058

-

smooth

80

CCW

O-pyrE-cysE

SA975

-

S. typhimurium

HfrK13

SA991

leuBCD39 ara-7

-

rough

78

CW

O-cysE-pyrE

SA977

-

S. typhimurium

HfrK8

SA991

leuBCD39 ara-7

-

smooth

76

CW

O-xyl-cysE

SA978

-

S. typhimurium

HfrK12

SA991

leuBCD39 ara-7

-

smooth

80

CCW

O-pyrE-cysE

SW1444

98

S. abony

HfrH1

SW1391

met-1151 aro-851 rpsL501

+

smooth

96-98

CW

O-pyrB-pro

SW1403

99

S. abony

HfrH2

SW1363 F(+)

met-1151 aro-851

+

smooth

53-60

CCW

O-purC-his

SH566

100

S. abony

HfrH3

SW1452

rpsL501

+

-

20-32

CCW

O-gal-pro

SH472

112

S. abony

HfrH4

SW1446

pur-259

+

-

90-94

CCW

O-metA-ilv

SH465

113

S. abony

HfrH5

SW1462

thi-53

+

rough

35-40

CW

O-his-purC

SH474

114

S. abony

HfrH10

SH650

thi-51

+

-

90-98

CCW

O-metA-ilv

SH462

115

S. abony

HfrH12

SH671

cys-1189

+

rough

50-60

CCW

O-aroC-his

(a) Genetic nomenclature is according to the system of Demerec et al., 1966. Genetics 54:61-76. (b) +, Fertility inhibition. Strains with this phenotype inhibit the fertility of F because of the Fin+ plasmid pSLT. -, fertility is not inhibited. (c) Phage sensitivity is an indicator of the lipopolysaccharide content of the cell. Some of the tests were done by B.A.D. Stocker at Stanford University. The smooth phage sensitivity phenotype, which indicates the presence of normal lipopolysaccharide in the cell wall, is that of sensitivity to the O-specific phages P22.c2, P22h.c2, and 9NA as well as to the rough- specific phages 6SR, Ffm, and Br60. Deviations from the smooth phage sensitivity pattern are called rough. Among the rough phenotypes, several subgroups can be distinguished. Methods for phage sensitivity tests and definitions of phage sensitivity phenotypes are provided by Wilkinson et al., 1972. J. Gen. Microbiol.70:527-554. (d) Min, inferred point of insertion of F into the chromosome; corresponds to minutes of transfer time in interrupted conjugation experiments with Hfr strains (see figure). CW, clockwise direction of transfer; CCW, counterclockwise direction of transfer. The earlier description of Hfr strains (Sanderson et al.,1972. Bacteriol. Rev. 36:608-637) showed a linkage map inferred to be 138 min in length; this was later revised to 100 min to correspond with the linkage map of E. coli K-12 (Sanderson, K.E. and J.R. Roth, 1988. edition VII, Microbiol. Rev. 52:485-532).

Figure notes:

Hfr strains of S. typhimurium (ouside the circle) and S. abony (inside the circle). The chromomsome is divided into100 units, starting at thr (unit 0), as shown by numbers on the circle. The point of origin and of transfer are indicated by arrows. For S. typhimurium, the strain number (e.g. SR305) is given, and the table gives the Hfr designation (HfrA) and the genotype of each strain. In those cases in which F is inserted into a known transduction linkage group, the Hfr is displayed on an arc outside the main circle. Where more than one Hfr strain number is shown in a gene interval (e.g. SA536, SA949 and SA977, shown within a bracket), the numbers represent independent isoltes for which the points of origin have not been proven different, though differences may exist. Not all strains with points of origin in the rfa genes (HfrK1 and HfrK2 at 79 min) are shown. For more detail see the the table above.

 

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